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Companies That Write Research Papers

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A Guide to Interesting Compare and Contrast Essay Topics

A Guide to Interesting Compare and Contrast Essay Topics Introducing Interesting Compare and Contrast Essay Topics You're able to attract books, movies or articles which are discussing exactly the same topic you're likely to approach in your essay. Describe two movies you have lately seen. For example an individual may opt for a topic like, life in the shoes of a werewolf. If you decide to compose a funny comparative essay then you must have good humor. For instance, you can consider a funny title or, as an alternative, you can imagine two aspects to look at that are so ridiculous that the humour is inevitable. Students who don't have a great awareness of humor might overdo it in their essay. Interesting Compare and Contrast Essay Topics - What Is It? Failure to organize the content well will be a big mishap and damage your grade. There is a range of interesting compare and contrast essay topics it's possible to select from you can describe laws you want to create, to offer you some adjustments to already existing laws, or to suggest to cancel a law which you don't like for certain factors. You should have valid evidence to back up your ideas together with proper analysis that your readers can see and follow all of the way down. Interesting Compare and Contrast Essay Topics and Interesting Compare and Contrast Essay Topics - The Perfect Combinat ion It truly is beneficial to realize that people usually take advantage of comparison along with comparison pondering in the massive quantity of life circumstances. In a lot of sections of text's primary body you are going to demonstrate different contrast or compare points. For instance, you might think of similarities or differences if we are purchasing a new MP3 player or picking a place to study English. There are similarities irrespective of location, like the company chosen to observe the movie, and the movie itself. If you're studying in the health care field, you might have to to compose many essays. Obviously, writing is among the top learning programs in any significant school institution. When it can be less painful to write on a well-known topic, with a little research and organization, a writer can make an essay on an assortment of subject areas if assigned. Students who excel in writing about such complex topic can have an opportunity to be enrolled into a num ber of the very best Art universities to come up with their abilities and talent. Thus, you are going to be in a position to define the most crucial facts. Most themes will require some particular expertise, meaning that the search for facts and statistics and extra details will take much moment. You must research your topic to select three claims. If you wish to be certain that you have an exceptional topic for your essay, you are able to look through the overall directions of our topics and hunt to learn more online. The Unexpected Truth About Interesting Compare and Contrast Essay Topics The critical reason many writers fail is they don't go further. Perhaps you thought it will be simple to select a topic by yourself, but the truth is that it is possible to end up sitting with a blank paper for a number of hours. The next thing to do is to begin writing. What's The Better Purchase Plasma TV Or LCD TV We reside in a huge choice. What Is So Fascinating About Interesting Compare and Contrast Essay Topics? Again, compare and contrast essays can cover nearly every topic and might pop up in an assortment of subject areas. It might be necessary to liaise with other people to recognize the good compare and contrast essay topics. Otherwise, you must have a look at a number of the easy compare and contrast essay topics on the many scientific innovations. There are lots of others persuasive compare and contrast essay topics regarding education if you are prepared to write about doing it. Life, Death, and Interesting Compare and Contrast Essay Topics A strong essay is going to have an opening paragraph that comes with a thesis statement and a concluding paragraph. It's possible to restate your thesis statement and point out a number of the arguments used over the whole essay that backs it up. You should research the selected topic and discover facts to contradict your first thesis. To compose a high-quality paper, you've got to understand how to s elect your topic properly and utilize it to create a practical outline.

External and Internal Environment Analysis - 2372 Words

Big 5 Sporting Goods External and Internal Environmental Analysis Big 5 Sporting Goods (Big 5) has operated within the sporting goods industry, predominantly in the western United States, for over 50 years (Hoovers, 2013). To understand the organizational structure and strategic path chosen by Big 5, it is critical to analyze various factors found within its external and internal environments. Big 5’s external environment includes remote, industry, and operating factors whereas the internal environment includes human capital resources and organizational structure factors. Ultimately these external and internal environmental factors provide opportunities and threats that help to develop and alter generic and grand strategies to meet Big†¦show more content†¦Ultimately understanding and analyzing the effect of each remote environmental factor will allow Big 5 to implement targeted strategies to capitalize on opportunities and minimize threats. Industry Environment Industry environment pertains to how an organization copes with the five basic forces that shape the competitive market in which it operates. How these forces relate to each other will determine the competitive nature of the industry and control how much profit can be made. In a perfectly competitive market, where new entrants are fairly consistent and lack barriers, the likelihood of long-term profits is low. This is seen in such industries as plastics and rubber. The five basic forces consist of suppliers, buyers, new entrants, substitutes, and industry competitors (Pearce, Robinson, 2011). Big 5 Sporting Goods industry environment starts with determining the possibility or ease of new entrants into the market. The ease of the market depends on how many barriers exist for entrants. There are six major sources of barriers, the first being economies of scale. Economies of scale allows Big 5 to purchase a large quantity of a product at a reduced per item cost. This in turn allows them to sell that product at a competitive price in the market. This creates a capital barrier for new entrants. An organization would need a significant amount of capital to take advantage of the economies of scale and stay priceShow MoreRelatedExternal and Internal Environment Analysis1429 Words   |  6 PagesRunning head: EXTERNAL AND INTERNAL ENVIRONEMNT External and Internal Environment Analysis Bharti Gupta University of Phoenix External and Internal Environment Analysis: Mayo Clinic The purpose of this paper is to analyze the External and Internal environmental factors of Mayo Clinic (MC). Mayo Clinic is a nonprofit worldwide leader in medical care, research and education for people from all walks of life (Mayo Clinic,  2011). Various types of external and internal factors affect theRead MoreExternal and Internal Environment Analysis1565 Words   |  7 PagesExternal and Internal Environmental Analysis This environmental analysis will provide a complete external environmental scan designed to provide RoyalBlu Invites with the tools needed to identify the organizations strength and weaknesses. Furthermore, including an assessment of the company resources. This analysis will assess the organization competitive position and possibilities of growth. An explanation of how the external environment affects RoyalBlu Invites structure and organizational performanceRead MoreInternal And External Environment Analysis Essay1260 Words   |  6 Pagesbusiness strategies, organizations must thoroughly analyze both the internal and external environments in which they operate (Hill, Jones, Schilling, 2014, p. 11). This analysis will enable them to pinpoint those elements that threaten their current and future profitability as well as those elements that offer the potential to increase current and future profitability (Hill, et al., 2014, pp. 44-45). The external environment analysis specifically looks at the industry within which a business functionsRead MoreExternal And Internal Environment Analysis835 Words   |  4 Pagesextensive external and internal environment analysis. When conducting external environmental analysis, a company must consider six factors. These factors include competition, economic growth, political trends, legal issues, advances in technology, and sociocultural trends (Ferrell Hartline, 2014). I have a strong interest in technology and gadgets. With that being the case, I chose the iPhone as the product and will analyze the six external environmental factors using that. The first external factorRead MoreExternal and Internal Environment Analysis Essay1101 Words   |  5 PagesExternal and Internal Environmental Analysis DSW is the leading footwear specialty retailer in the United States operating 305 shoe stores in 39 states as of January 30, 2010. The shoe warehouse offers a wide assortment of better-branded dress, casual and athletic footwear for women and men, as well as assessories. The company’s main focus is to create a distinctive shopping experience that satisfies both the rational and emotional needs of DSW customers by offering a vast, exciting assortment ofRead MoreAnalysis of Subways internal and external environments2247 Words   |  9 PagesIntroduction This essay will analyse the organisation Subway’s internal and external environments and their impacts on this organisation. This will include a swot analysis on resources and capabilities which are a part of the internal environment and on customers, suppliers, competitors, pressure groups, economic, political, technological, natural environment and emerging trends in the external environment. â€Å"A SWOT Analysis is a useful technique for understanding your Strengths and Weaknesses, andRead MoreInternal Analysis And External Environment Assessment1869 Words   |  8 Pages1) Internal Analysis and External Environment Assessment Industry’s Dominant Economic Characteristics Market Size: Annual sales revenue for 2014 according to NASDAQ $68,775,000. Scope of Competitive Rivalry: Global for Comcast and NBC Universal. Market Growth Rate: According to NASDAQ 11.21% over the next five years. Stage in Life Cycle: Residential Area = Mature, Small and Medium Business Area = still growing and the Enterprise (Ethernet Area) = Early development rapid growth. NumberRead MoreInternal Analysis And External Environment Assessment1865 Words   |  8 PagesI. Internal Analysis and External Environment Assessment Industry’s Dominant Economic Characteristics Market Size: Annual sales revenue for 2014 according to NASDAQ $68,775,000. Scope of Competitive Rivalry: Global for Comcast and NBC Universal. Market Growth Rate: According to NASDAQ 11.21% over the next five years. Stage in Life Cycle: Residential Area = Mature, Small and Medium Business Area = still growing and the Enterprise (Ethernet Area) = Early development rapid growth. NumberRead MoreCompany s External And Internal Environment Analysis1387 Words   |  6 PagesExecutive summary The main purpose of this paper is through the company s external and internal environment analysis to evaluate the company in the industry and future developments. Assess the external environment mainly through PESTLE Analysis and Porter s 5 Forces. Strategic Recourses Capability Analysis for internal environmental assessment is conducted. Finally, the company somehow SWOT analysis, concluded †¢Ã¢â‚¬ ¢Ã¢â‚¬ ¢Ã¢â‚¬ ¢ This article mainly refer to the company s annual financial reports, industryRead MoreSwot Analysis : The Internal And External Environment Of A Firm1658 Words   |  7 Pages1. SWOT analysis is a technique to analyze the internal and external environment of a firm. What are its advantages and disadvantages? SWOT provides a useful starting point for improving firms and better positioning them for success. However, SWOT is a limited technique with at least 4 problems. First, SWOT may identify strengths, but these do not always translate into competitive advantages. To generate competitive advantages, firms have to understand the source of their strengths and focus resources

Pursuit of the American Dream in Great Gatsby and Death...

Comparing the Pursuit of the American Dream by Jay Gatsby and Willy Loman (Essay outline also included in the word count.) People from all around the world have dreamed of coming to America and building a successful life for themselves. The American Dream is the idea that, through hard work and perseverance, the sky is the limit in terms of financial success and a reliable future. While everyone has a different interpretation of the American Dream, some people use it as an excuse to justify their own greed and selfish desires. Two respected works of modern American literature, The Great Gatsby and Death of a Salesman, give us insight into how the individual interpretation and pursuit of the American Dream can produce tragic†¦show more content†¦Despite receiving little payoff for his efforts, he regularly gives extravagant parties to impress others, always half expecting [Daisy] to wander [in] some night (84). Similar to Gatsby, Willy Loman from Arthur Millers Death of a Salesman, bases his American Dream on the idea that public acceptance would bring him wealth. Willy believes that appearing well liked makes him successful. Willy has based his life on the idea that if you are well liked, you will never want (Miller 33). Moreover, he believes his sons appearance will make them successful because they are both built like Adonises (33). Furthermore, Willys obsession with money leads him to equate the value of an individual with their financial worth. Willy idealized his older brother Ben because he is rich (41). Willy, reflecting on his own worth, concludes you end up worth more dead than alive (98). Finally, Willy thinks that being a popular and successful businessman will win him the love of his wife and children. Willy lies to his family, by telling his sons [if there is] one thing boys: I have friends(31). He also exaggerates terribly by telling his wife he sold five hundred gro ss in Providence and seven hundred gross in Boston (35). In his final imagined discussion with his brother Ben, Willy concludes that by taking his own life he will finance his sons business venture, and [Biff will] worship me for it (135). Although JayShow MoreRelatedDeath of a Salesman Compared to the Great Gatsby Essay613 Words   |  3 PagesComparing Death of a Salesman to The Great Gatsby In the search for the American dream many things can be lost, this is reflected in the novel The Great Gatsby and the movie Death of a Salesman. Both of these works demonstrate the lengths that some people will go to in order to achieve the stereotypical life of a rich, successful and powerful American, which is often referred to as the American dream. Death of a Salesman by Arthur Miller illustrates how the character Willy will stop at nothing toRead MoreThe American Dream in Death of a Salesman by Arthur Miller, and The Great Gatsby by F. Scott Fitzgerald1096 Words   |  5 PagesThe American Dream in Death of a Salesman by Arthur Miller, and The Great Gatsby by F. Scott Fitzgerald In a majority of literature written in the 20th century, the theme of the American Dream has been a prevalent theme. This dream affects the plot and characters of many novels, and in some books, the intent of the author is to illustrate the reality of the American Dream. However, there is no one definition of the American Dream. Is it the right to pursue your hearts wish,Read MoreGatsby And Death Of A Salesman Analysis1003 Words   |  5 PagesJay Gatsby in F. Scott Fitzgerald’s The Great Gatsby and Willy Loman in Arthur Miller’s Death of a Salesman are both American men in pursuit of the American Dream which is, to acquire wealth, success, and prestige. This quest for money drives modern-day America, but behind our perpetual urge to consume and possess lays a grim motive. It is human awareness of mortality and the subsequent desire to prove we are special and somehow resistant to death that fuels the longing for wealth and possessionsRead MoreThe Death Of A Salesman By Arthur Miller1497 Words   |  6 PagesThe Death of a Salesman is a novel by Arthur Miller. It illustrates how the protagonist Willy stops at nothing to achieve what he believes is the American dream in spite of encountering many costs. Willy is, however, n ot able to achieve his American dream, because he is very stubborn. The Great Gatsby is a novel written by F. S. Fitzgerald and is also about the pursuit of the American dream. The two novels detail the downfall of their respective protagonists in their quest to achieve the elusiveRead MoreThe Great Gatsby And The Death Of A Salesman1427 Words   |  6 Pages In both The Great Gatsby and The Death of a Salesman, the pursuit of the American Dream is a destructive force that is evident throughout the texts because neither Gatsby nor Willy understand their own limitations. Willy Loman and Gatsby are characters fascinated and easily dominated by the American dream that destroys them. Their dreams come from an illusionary past and that are both based outside of their own selves. Gatsby wanted to rewind to his past, while Willy attempted to create a descentRead MoreWilly Loman, Jay Gatsby, and the American Dream Essay736 Words   |  3 PagesGatsby and Loman My own life’s dream is to have a career in which I can surround myself in music. I will not let anything stand between my dream and I; however, I will never allow my dream to come between myself and my loved ones, or my reality. Many have already fallen victim to the pursuit of the American Dream – a fantastic projection of life that varies based upon its dreamer. However, the road to the American Dream, if followed blindly, can lead to nothing more than a dead end. In The GreatRead MoreFailure Of The American Dream In The Writings Of F. Scott Fitzgerald, Zora Neale Hurston, And August Wilson1418 Words   |  6 Pageswill define the failure of the †American Dream† in the writings of F. Scott Fitzgerald, Arthur Miller, Zora Neale Hurston, and August Wilson. Fitzgerald’s account of the Jay Gatsby s rise to fame in the 1920s defines the failure of financial success as part of the American Dream. Gatsby will eventually die due to his excessive greed, wh ich is not unlike the emotional death of Willy Loman as he fails to become a successful salesman in Author Miller’s Death of a Salesman. More so, Hurston’s depictionRead MoreAmerican Dream Derailed in The Great Gatsby and Death of a Salesman1486 Words   |  6 PagesThe American dream originated when immigrants came to America searching for new opportunities and a better life. In the early 1900’s all people could do is dream; however, those dreams gave many different meanings to the phrase â€Å"American dream†, and for the most part, wealth and hard work play a very large role in the pursuit of â€Å"the dream†. In F. Scott Fitzgerald’s novel, The Great Gatsby, and Arthur Miller’s drama, Death of a Salesman, both protagonists, Jay Gatsby and Willy Loman, are convincedRead MoreThe Great Gatsby And Death Of A Salesman1241 Words   |  5 Pagesambition has been so prevalent in literature. It is the dominant theme in The Great Gatsby b y F.Scott Fitzgerald, William Shakespeare s Macbeth, Death of a Salesman by Arthur Miller and The Wolf of Wall Street by Jordan Belfort. In this essay, I will discuss the two ways over ambition can cause an individual s downfall. The first is setting unattainable goals which we can see in The Great Gatsby and Death of a Salesman. The second is trying to achieve your goals unethically, seen in Macbeth andRead MoreThe Great Gatsby By F. Scott Fitzgerald1346 Words   |  6 Pagesinvented standard that we have all come to know as ‘The American Dream’. The pursuit of this ever so highly held ideal not only drives many a character forward, but in some cases over the edge of sanity or even to their untimely deaths. This in a way makes ‘The Dream’ some sort of green eyed monster lurking in the darkest of corners found in the human mind. Making the pursuit of dreams almost as dangerous as say a high speed police pursuit on a crowded highway, the wrong side of a cro wded even. For

Don Quixote Reality vs. Illusion free essay sample

An analytical paper which compares and contrasts the theme of reality vs. illusion in Don Quixotes world. A paper which shows how reality and illusion are interwoven not only throughout the story line of Don Quixote exploits, but how author, Miguel de Cervantes, even leaves the true authorship of this tale in question, suggesting that he is merely translating the story from the original writings of Cide Hamete. The paper discusses the madness of Don Quixote who sees people and objects in his everyday life as the counterparts in his knights and damsels world of chivalry, and how Don Quixote charging the windmills has become a classic scene and now serves to symbolize bravery in vain. Cervantes begins Part Two of Don Quixote again referring to the writings of Cide Hamete, thus, lending credence to both, Don Quixote and Hamete as real historic characters (Cervantes, 529). This further adds confusion between reality and fantasy. We will write a custom essay sample on Don Quixote: Reality vs. Illusion or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page Throughout the novel there are countless scenes crossing the line of reality and illusion. The deathbed scene is perhaps the most poignant, for at the end, even though he himself had given up his chivalrous life and rejoined society, the others around him were still holding on to his fantastic world of knights and damsels in distress. But Don Quixote speaks, I am no longer Don Quixote of La Mancha, but Alonso Quixana.

Antiproliferative effect of silver nanoparticles free essay sample

Antiproliferative effect of silver nanoparticles synthesized using amla on Hep2 cell line BY stanleY00086 Asian Pacific Journal of Tropical Medicine (2012)1-10 Contents lists available at ScienceDirect Asian Pacific Journal of Tropical Medicine journal homepage:www. elsevier. com/locate/ap]tm Document heading dot: Antiproliferative effect of silver nanoparticles synthesized using amla on Hep2 cell line Fathima Stanley Rosarinl , Vadivel Arulmozhil , Samuthira NagaraJan2, Sankaran Mirunalinil* Department of Biochemistry and Biotechnology, Annamalai University, Annamalai nagar-608 002, Tamilnadu, India Department of Chemistry, Annamalai Results: PE-AgNPs was synthesized and confirmed through kinetic behavior of NPs. The shape of PE-AgNPs was spherical and ubic since it was agglomerated, and the nanoparticle surface was complicated. Average particle size distribution of PE-AgNPs was found to be 188 nm. Potent biomolecules of P. emblica such as polyphenols were capped with AgNPs and reduced its toxicity. In cytotoxicity assay the concentration in which the maximum number of cell death was 60 fig/mL and 50 fig/mL for P. We will write a custom essay sample on Antiproliferative effect of silver nanoparticles or any similar topic specifically for you Do Not WasteYour Time HIRE WRITER Only 13.90 / page emblica (alone) and AgNPs, respectively and IC50 values were fixed as 30 fig/mL and 20 fig/mL. ROS generation, apoptotic morphological changes, mitochondrial depolarization, DNA damage and oxidative stress was observed as more in AgNPs treated cells than in P. mblica (30 g/mL) (alone) treated cells and 5-FU treated cells gave similar result. Conclusions: The results suggest that the AgNPs are capped with biomolecules of amla enhanced cytotoxicity in laryngeal cancer cells through oxidative stress and apoptotic function on Hep2 cancer cells. Keywords: Amla AgNPS Oxidative stress Cytotoxicity Antiproliferation 1. Introduction Cancer is considered as one of the most deadly disease in the world with high mortality. Since there are many cancer therapies available, chemotherapy has become an integral component of cancer treatment for most cancers. In the area f oncology drug discovery, conventional chemotherapeutic agents still exhibit poor specificity in reaching tumor tissue and are often restricted by dose-limiting toxicity. The combination of developing controlled-release technology and targeted drug delivery may provide a more efficient and less harmful solution to overcome the limitations in *Corresponding author: Dr. S. Mirunalini, assistant professor, Depatment of Biochemistry, Annamalai University, Annamalai nagar 608 002, Tami nadu, India. Tel: Fax: conventional chemotherapy. R ecent interest has been are capable of controlling the release of chemotherapeutic gents directly inside cancer cells[l]. Nanomaterials are expected hopefully to revolutionize the cancer diagnosis and therapy. Nanoscale particles decorated with multiple functionalities are able to target and visualize tumor site via an imaging technology, thereby allowing for the early detection of cancer. Furthermore, intelligent nanosystems can be constructed as controlled delivery vehicles which are capable of delivering anticancer drugs to a predetermined site and then releasing them with a programmed rate, which can improve therapeutic efficacy[2]. In inorganic nanoparticles, metal nanoparticles have eceived considerable attention in recent years because of their unique properties and potential applications in Fathima Stanley Rosarin et al. /Asian Pacific Journal of Tropical Medicine (2012)1-10 catalysis, photonics, optoelectronics, biological tagging and pharmaceutical applications. A number of approaches are available for the synthesis of silver nanoparticles (AgNPs). For example, silver ions are reduced by chemical, electrochemical, radiation, photochemical methods, Langmuir-Blodgett and biological techniques[3-9]. Among these methods, biological synthesis is a good way to fabricate benign nanostructure aterials. Biological technique is less toxic and eco friendly, in the synthesis of nanoparticles capping agents are used. Capping agent is absorbed by nanoparticles. They are usually organic molecules, and used to aid stabilization of nanoparticles. Silver nanoparticles were synthesized by various plant materials as capping agents such as papaya[10] and neem[11]. Polyphenols of Phyllanthus emblica (P. emblica) includes hydrolysable tannins, flavonoids, alkaloids, gallic acid, ellagic acid and quercetin [1 2], and this plant exhibits antioxidant[13], adaptogenic[14], and hepato-protective action[1 5]. Due to the existence of the conjugated ring structures and hydroxyl groups, many phenolic compounds have the potential to function as antioxidants by scavenging superoxide anion[16] and singlet oxygen[17]. Metal nanoparticles can be synthesized in the average size of more than 50 nm, therefore their large surface area has the ability to carry a relatively high drug dose. Functionalizing the surface of conventional metallic nanoparticles like gold and silver to carry drugs is under investigation. investigate the anticancer effect of PE-AgNPs and drug delivery efficacy of Ag nanoparticles on Hep2 (laryngeal pidermoid carcinoma, a common malignant tumor of head and neck)[18] cell line which has not been previously studied. The biological applications of silver nanoparticles (AgNPs), antimicrobial properties in particular have been widely studied[19]. AgNPs are known to be cytotoxic to both normal and cancer cells in mammals [20] and the modes of interactions of AgNPs have been investigated in different prokaryotic and eukaryotic systems [21-23]. Since nanoparticles ( NPs) are more biocompatible than the conventional therapeutics, they are exploited for drug encapsulation and delivery[24]. It has been stressed over he years that size reduction of NPs play an important role in improving their bioavailability as well as compatibility for therapeutical applications in diseases like cancer[25]. Silver nanoparticles (AgNPs) have a great potential in cancer management because it selectively involved in disruption of the mitochondrial respiratory chain by AgNPs leading to production of ROS and interruption of ATP synthesis, which in turn cause DNA damage[26,21]. Based on the conflicting results, here is an urgency to evaluate cytotoxicity and apoptotic properties of PE-AgNPs on hep2 cells. In order to study the properties of PE-AgNPs to induce apoptosis n cancer cells, it was compared with a standard reference drug 5-flourouracil (5-FU) and we also compared P. emblica fruit extract (alone) with its encapsulated AgNPs. For this study, we employed a well characterized AgNPs to access its apoptotic function via its cytotoxicty and oxidative stress. Dose was fixed and applied in the Hep2 cells by the cytotoxicity test. Based on this study and our ability to access the interaction and interference with a wide range of biological functions, PE-AgNPs employed in the present study provide a unique opportunity to investigate oxidative stress, intracellular ROS generation, apoptotic bodies and poptotic DNA fragmentation, and mitochondrial membrane potential and toxicity in human laryngeal carcinoma cells (Hep2 cell line). Though the AgNPs induces mitochondrial mediated apoptosis[27], we have made an attempt to cap the AgNPs with potent biomolecules of P. mblica and we assessed the toxicity and examined the apoptotic function of PE-Ag NPs which comprised of biomolecules of P. emblica and silver precursors. 2. 1 . Silver nanoparticles The silver nanoparticles were synthesized using previously published procedure in which AgNPs were produced through ion reduction and subsequent stabilization using aqueous xtract of P. emblica fruits. In this method P. emblica pericorps were initially rinsed thrice in distil led water and dried on paper toweling. About 25 g of fruit were cut into fine pieces and boiled with 100 mL sterile distilled water for 5 minutes and filtered through Whatman No. l filter paper twice. The filtrate was stored at 4 C and used for the present study. About 10 mL of aqueous fruit extract was added into the 100 mL aqueous solution of 1 mM AgN03 (AR)[28]. The 100 mL of 1 mM silver nitrate solution was reduced using 10 mL of P. emblica extract at room temperature within 10 min. Ag ion reduction was monitored by measuring the UV-vis spectrum of the reaction medium at various time intervals (5 min to 78 h) in room temperature. The pellet of AgNPs obtained after centrifugation was air dried and mixed with KBr and the KBr-AgNPs pellet was subjected to FTIR to ensure the formation of silver nanoparticles with encapsulation of biomolecules of P. emblica. A scanning electron microscope was used to record the micrograph images of synthesized AgNPs, the particle size distribution of AgNPs was evaluated using dynamic light scattering measurements. These well characterized silver nanoparticles ere further used for cytotoxicity and oxidative stress on cancer cells. 2. 2. Cell culture The human laryngeal carcinoma cell line ( Hep2) was purchased from National Centre for Cell Science, Pune, I ndia. T he cells were cultured as monolayer in MEM supplemented with 10% FBS, 1% glutamine and 100 IJ/mL penicillin-streptomycin at 37 C in 5% C02 atmosphere, stocks were maintained in 25 cm2 tissue culture flasks. A stock solution of PE (1 mg/mL) and PE-AgNPs (1 mg/mL) was prepared in 0. 5% dimethyl sulphoxide (DMSO) (w/v) and stored at 4 C further dilution was made in culture media to obtain the desired oncentrations. The final concentrations of DMSO in the culture medium were not more than 0. 01% (wv). 0. 01% DMSO was used as a sham control. C ells were treated with different concentration of PE and PE-AgNPS (1, 5, 10, 20, 30, 40, 50 fig,t-nL) and the cytotoxicity was observed by (3-4, 5-dimethyl thiaz012yl)-2, 5-di phenyl tetrazolium bromide (MTT) assay. MTT assay was first proposed by mossmon[29]. IC50 value was calculated and optimum dose of PE and PE-AgNPs was fixed in this assay for further study. It is a colorimetric assay for measuring the activity of enzymes that reduce MTT to purple color. Formazan product is directly proportional to viable cells. 10 GL of MTT solution (5 mg/mL in PBS) was added to each culture well after 24 hours of incubation with PE and PE-AgNPs treatment. The color was allowed to develop for additional 4 hours incubation. An equal volume of DMSO was added to stop the reaction and to solubilize the blue crystals. The absorbance was taken using UV-visible spectrophotometer (Elico SLl 59, India) at a wavelength of 570 nm. OD value was subjected to sort-out percentage of viability by using the following formula, OD value of experimental samples. Percentage of cell viability= OD value of experimental sample (AgNPs) OD value of experimental control (untreated) 2. 4. Cell treatment The Hep2 cells were treated with PE and PE-AgNPs in the following concentrations as revealed by MTT assay. They are Group l- Control (untreated cancer cells), Group ll- Hep2 cells (30 Gg/mL PE), Group Ill Hep2 cells + 20 fig/mL PE-AgNPs and Group IV Hep2 cells + 30 Gg/mL 5-FU. After treatment they were incubated at 37 C in 5% C02 incubator after 24 h incubation. The cells were harvested by trypsinization for further experiments. . 5. Reactive oxygen species generation R eactive oxygen species was assessed following the rocedure described by J esudason et al [30]. C ells were seeded in 96 well plate and incubated with PE extract (30 figmL), PE-AgNPS (20 fig,t-nL) and 5-FU (30 for 24 h. A fter incubation, fluorescent dye 2 , 7 dichlorfluorescein-diacetate (DCFH- DA) a non-fluorescent probe that can penetrate into the intracellular matrix of cells, wh ich were then kept in incubator for 30 min. Then the cells were washed with PBS to remove the excess dye. . 6. Mitochondrial membrane potential Mitochondrial membrane potential was evaluated following the procedure described by B hosle et al [31] using the Rhodamine-123 (Rh-123) which is a lipophilic cationic dye, 3 highly specific for mitochondria. The cells were seeded in 96 well plate and treated with PE (30 fig/mL), PE-AgNPs (20 fig/mL) and 5-FIJ (30 fig/mL) and incubated for 24 h. A fter incubation of the cells, fluorescent dye R h- 123 (5 mmol/L) was added to the cells and kept in incubator for 30 min. Then the cells were washed with PBS and viewed under fluorescent microscope using blue filter. 2. 7. Apoptotic morphological changes The apoptotic bodies which were the result of treatment with PE-Ag NPs was assessed by the method of Lakshmi et al[32]. Apoptotic nuclei exhibits typical changes such as nuclear condensation and fragmentation were stained by AO/EtBr to know the dead apoptotic cells. Cells were treated with PE (30 Gg/mL), PE- AgNPS (20 fig/TlL) and 5-FIJ (30 fig/mL), and incubated in C02 incubator for 24 h. The cells were fixed in methanol: glacial acetic acid (3:1) for 30 min at 37 C. The cells were washed with PBS and stained in 1:1 ratio of AO/EtBr, stained cells were immediately washed and viewed under a fluorescent microscope with a magnification of 40x. 2. 8. Apoptotic DNA fragmentation A poptotic DNA fragmentation is a key feature of rogrammed cell death and also occurs in certain stages of necrosis. DNA damage was estimated by agarose gel electrophoresis of DNA fragmentation[33]. Hep2 cells were treated with PE ( 30 g/m L), PE A g NP s (20 g/m L) and 5-FU (30 Gg/mL). Treated and untreated cells were collected by centrifugation at 3 000 rpm for 15 min at 4 C. The cell pellet was suspended in cell lysis buffer (Tris Hcl 10 mmol/L pH 7. 4, Triton-xlOO, 0. 5%) and kept at 4 C for 20 minutes. The supernatant was incubated with RNAase of 40 fig/L at 37 for 1 h then incubated with proteinase K 40 Gg/L at 37 for 1 h. To he final aqueous phase 40 GL of 3. 5 M ammonium acetate was added, to this ice cold isopropanol was added and centrifuged at 25 000 rpm for 15 min and dried. After drying, DNA was dissolved in TE buffer and separated by 2% agarose gel electrophoresis at 100 V for 50 min and the DNA Damage was analyzed by gel documentation (alpha innotech image analyzer). 2. 9. Lipid peroxidation Accumulation of lipid peroxides in the cell is associated cellular stress which leads to cancer cell death. The cells were harvested by trypsinization, the cell pellet obtained was suspended in PBS . T he suspension was taken for iochemical estimations. The level of lipid peroxidation was determined by analyzing TBA-reactive substances (TBARS)[34]. The pink chromogen formed by the reaction of 2-TBA with breakdown products of lipid peroxidation was Figure 1 . Solution of fruit extract, AgNO 3 solution and Ag nanoparticles (From left to right). 0. 200 The statistical analysis was done among the experimental groups with control and normal groups using SPSS software Version 16 (SPSS Inc. , Chicago, IL, USA). The One-way ANOVA was done for expressing experimental significance of the present study. Statistical significance was accepted t a level of P 0. 05. Abs. 3. 1 . Nanoparticle synthesis and characterization 0. 050 When aqueous extract of P. mblica was added to silver nitrate solution and stirred for 1 h, the resultant solution was brownish orange (F igure 1 ).